Anticorps Recombinant de lapin anti-FAM116A

FAM116A Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240486B9

N° de cat : 83473-3-RR

Synonymes

DENND6A, Protein FAM116A, Protein DENND6A, DENN domain-containing protein 6A, 240486B9

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Galerie de données de validation

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  WB analysis of PC-3 using 83473-3-RR

  PC-3 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83473-3-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 83473-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FAM116A Recombinant antibody (83473-3-RR, Clone:240486B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83473-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83473-3-RR against Human FAM116A were performed. The affinity constant is 1.43 nM.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83473-3-RR cible FAM116A dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FAM116A Protéine recombinante Ag22052
• Nom complet: family with sequence similarity 116, member A
• Masse moléculaire calculée: 608 aa, 70 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC040291
• Symbole du gène: FAM116A
• Identification du gène (NCBI): 201627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM116A has DENN-related domains, which are required for cell migration.

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