Anticorps Recombinant de lapin anti-FAM136A

FAM136A Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241433D12

N° de cat : 84150-2-RR

Synonymes

241433D12, Protein FAM136A

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Galerie de données de validation

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  WB analysis using 84150-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84150-2-RR (FAM136A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 84150-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 84150-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84150-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FAM136A antibody (84150-2-RR, Clone: 241433D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U2OS using 84150-2-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug FAM136A Recombinant antibody (84150-2-RR, Clone:241433D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84150-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84150-2-RR against Human FAM136A were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellule Hela, cellule U2OS, cellules Jurkat, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84150-2-RR cible FAM136A dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FAM136A Protéine recombinante Ag34058
• Nom complet: family with sequence similarity 136, member A
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC014975
• Symbole du gène: FAM136A
• Identification du gène (NCBI): 84908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM136A is an evolutively conserved nuclear gene encoding a mitochondrial protein whose specific function is unknown, but which is commonly found in neurosensory epithelial cells, where it plays a role in the electron transport chain of respiration (PMID: 37461313). FAM136A, with a molecular mass of 16-kDa, is normally expressed in the cytoplasm and has been linked to familial Meniere's disease.

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