Anticorps Polyclonal de lapin anti-FAM171A2

• Phare
• Validé par KD/KO

FAM171A2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 20836-1-AP

Synonymes

Protein FAM171A2

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Galerie de données de validation

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  WB analysis of mouse liver using 20836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20836-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20836-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20836-1-AP cible FAM171A2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM171A2 Protéine recombinante Ag14909
• Nom complet: family with sequence similarity 171, member A2
• Masse moléculaire calculée: 826 aa, 87 kDa
• Poids moléculaire observé: 65-75 kDa
• Numéro d’acquisition GenBank: BC030200
• Symbole du gène: FAM171A2
• Identification du gène (NCBI): 284069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM171A2 is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases. In addition, FAM171A2 was considerably expressed in the vascular endothelium and microglia, which are rich in Progranulin (PGRN) (PMID: 33087363).

Publications

Species	Application	Title
mouse	WB,IHC,IF	Sci Adv; The FAM171A2 gene is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases.; Authors - Wei Xu ; KD Validated

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