Anticorps Polyclonal de lapin anti-FAM20C

FAM20C Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 25395-1-AP

Synonymes

Dentin matrix protein 4, DMP 4, DMP4, DMP-4, EC:2.7.11.1

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Galerie de données de validation

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  IHC staining of human kidney using 25395-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25395-1-AP (FAM20C Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 25395-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25395-1-AP (FAM20C Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 25395-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25395-1-AP (FAM20C Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu rénal humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Informations sur le produit

25395-1-AP cible FAM20C dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM20C Protéine recombinante Ag22061
• Nom complet: family with sequence similarity 20, member C
• Masse moléculaire calculée: 584 aa, 66 kDa
• Numéro d’acquisition GenBank: BC040074
• Symbole du gène: FAM20C
• Identification du gène (NCBI): 56975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM20C, also named as Golgi-enriched fraction casein kinase or Dentin matrix protein 4, is a 584 amino acid protein, which belongs to the FAM20 family. FAM20C is widely expressed. FAM20C as a Calcium-binding kinase that phosphorylates the caseins and several secreted proteins implicated in biomineralization, including the secretory calcium binding phosphoproteins (SCPP).

Publications

Species	Application	Title
monkey	WB	Nat Commun; Altered sulfation status of FAM20C-dependent chondroitin sulfate is associated with osteosclerotic bone dysplasia; Authors - Toshiyasu Koike
rat	WB,IHC	FASEB J; Fam20C-mediated phosphorylation of osteopontin is critical for its secretion but dispensable for its action as a cytokine in the activation of hepatic stellate cells in liver fibrogenesis.; Authors - Elena Tibaldi
mouse	WB	JCI Insight; 1,25-Dihydroxyvitamin D3 regulates furin-mediated FGF23 cleavage; Authors - Han Xie
mouse	IHC	Gene; Knocking out FAM20C in pre-osteoblasts leads to up-regulation of osteoclast differentiation to affect long bone development; Authors - Lili Jiang
human	WB	Front Immunol; FAM20A: a potential diagnostic biomarker for lung squamous cell carcinoma; Authors - Yalin Zhang
human	WB	Int J Biol Macromol; Discovery of a novel Fam20C inhibitor for treatment of triple-negative breast cancer; Authors - Yingying Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Doug (Verified Customer) (05-01-2019)

Does not work. This antibody is garbage. Don't waste your money.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: MDA-MB231 a line that I can readily detect FAM20C by MS

FAM20C Antibody Western Blot, validation (1/1000 dilution) in MDA-MB231 a line that I can readily detect FAM20C by MS (Cat no:25395-1-AP)

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