Anticorps Recombinant de lapin anti-FAM38B

FAM38B Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240406E5

N° de cat : 83488-4-RR

Synonymes

Piezo2, Transmembrane protein C18orf30, C18orf30, 240406E5

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Galerie de données de validation

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  WB analysis of HeLa using 83488-4-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 83488-4-RR (FAM38B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 83488-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Fam38b Recombinant Antibody (83488-4-RR, Clone:240406E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83488-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83488-4-RR against Human FAM38B were performed. The affinity constant is 3.8 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83488-4-RR cible FAM38B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FAM38B Protéine recombinante Ag24528
• Nom complet: family with sequence similarity 38, member B
• Masse moléculaire calculée: 318 kDa
• Poids moléculaire observé: 250-310 kDa, 80 kDa
• Numéro d’acquisition GenBank: AB527139
• Symbole du gène: FAM38B
• Identification du gène (NCBI): 63895
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM38B, also named as PIEZO2, is a mechanosensitive, rapidly inactivating (RI) ion channel which is open and converts the mechanical stimulus signals into bioelectrical signals after stimulated by mechanical signals. FAM38B has been recently identified in dorsal root ganglion (DRG) neurons to mediate tactile transduction. It plays an important role in the biological process, maintaining cell metabolism and cell migration. Loss-of-function mutations in the human FAM38B gene cause an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis.The 80 kDa band detected by SDS-PAGE can be caused by alternative splicing (PMID: 34335288, 37227654).

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