Anticorps Polyclonal de lapin anti-FAM3C

FAM3C Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14247-1-AP

Synonymes

FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C

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Galerie de données de validation

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  WB analysis using 14247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14247-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14247-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14247-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14247-1-AP (FAM3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14247-1-AP (FAM3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HEK-293, cellules HepG2, cellules HT-29, tissu cardiaque humain, tissu cérébral humain
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below

Informations sur le produit

14247-1-AP cible FAM3C dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM3C Protéine recombinante Ag5516
• Nom complet: family with sequence similarity 3, member C
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC046932
• Symbole du gène: FAM3C
• Identification du gène (NCBI): 10447
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.

Publications

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
human	WB	J Proteome Res; Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.; Authors - Loei Hendrick H
human	WB	Diagn Pathol; Prognostic significance of FAM3C in esophageal squamous cell carcinoma.; Authors - Ying-Hui Zhu
human	IHC	Int J Clin Exp Pathol; Overexpression of FAM3C protein as a novel biomarker for epithelial-mesenchymal transition and poor outcome in gastric cancer.; Authors - Shuai Yin
human,mouse	WB,IHC	Int J Biol Sci; A Novel ER Stress Mediator TMTC3 Promotes Squamous Cell Carcinoma Progression by Activating GRP78/PERK Signaling Pathway; Authors - Hongyu Yuan
human	IHC	Sci Rep; FAM3 family genes are associated with prognostic value of human cancer: a pan-cancer analysis; Authors - Qing-Tai Dong