Anticorps Polyclonal de lapin anti-FANCL

FANCL Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 19187-1-AP

Synonymes

PHF9, Fanconi anemia-associated polypeptide of 43 kDa, Fanconi anemia group L protein, FAAP43, EC:2.3.2.27

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Galerie de données de validation

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  WB analysis of human brain using 19187-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19187-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19187-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19187-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 19187-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19187-1-AP (FANCL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293, cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19187-1-AP cible FANCL dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FANCL Protéine recombinante Ag6192
• Nom complet: Fanconi anemia, complementation group L
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 41-43 kDa
• Numéro d’acquisition GenBank: BC054517
• Symbole du gène: FANCL
• Identification du gène (NCBI): 55120
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.

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