Anticorps Polyclonal de lapin anti-FASTKD3

• Phare
• Validé par KD/KO

FASTKD3 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 18392-1-AP

Synonymes

FAST kinase domains 3, FASTKD3

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Galerie de données de validation

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  WB analysis of HeLa using 18392-1-AP

  WB result of FASTKD3 antibody (18392-1-AP, 1:200) with HeLa and 293 cell lysate by Maciej Szewczyk University of Warsaw.

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  WB analysis of HepG2 using 18392-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 18392-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 18392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of transfected cells using 18392-1-AP

  IF result of anti-FASTKD3 (18392-1-AP, 1:200) with transfected COS7 cell by Dr. Nancy Kedersha.

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  IF Staining of HepG2 using 18392-1-AP

  Immunofluorescent analysis of HepG2 cells using 18392-1-AP (FASTKD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18392-1-AP

  Immunofluorescent analysis of HeLa cells using 18392-1-AP (FASTKD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules transfectées, cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

18392-1-AP cible FASTKD3 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FASTKD3 Protéine recombinante Ag13083
• Nom complet: FAST kinase domains 3
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC001295
• Symbole du gène: FASTKD3
• Identification du gène (NCBI): 79072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAST kinase domain-containing protein 3(FASTKD3) belongs to FASTKD family.FASTKD3 is an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration. The expression of endogenous FASTKD3 is very weak. This antibody detects the c-terminal of FASTKD3.

Publications

Species	Application	Title
human	WB	J Biol Chem; Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.; Authors - Erik Boehm ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erik (Verified Customer) (02-25-2021)

We used the antibody at 1:500 dilution, and it produced several bands, including the expected one at 75kda approx (as per UniProt annotation). The unexpected bands were, however, more intense than the expected one.

• Applications: Western Blot
• Cell Tissue Type: MIAPACA2 cell line

FASTKD3 Antibody Western Blot validation ( dilution) in MIAPACA2 cell line (Cat no:18392-1-AP)