Anticorps Monoclonal anti-Fibulin 5

• Phare
• Validé par KD/KO

Fibulin 5 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

2A5C8

N° de cat : 60081-1-Ig

Synonymes

FBLN5, 2A5C8, Dance, Developmental arteries and neural crest EGF-like protein, EVEC

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Galerie de données de validation

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  WB analysis of MCF-7 using 60081-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60081-1-Ig

  WB result of Fibulin 5 antibody (60081-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fibulin 5 transfected MCF-7 cells.

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  WB analysis of human placenta using 60081-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 60081-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 60081-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 60081-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 60081-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 60081-1-Ig(Fibulin 5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque humain, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

60081-1-Ig cible Fibulin 5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fibulin 5 Protéine recombinante Ag2859
• Nom complet: fibulin 5
• Masse moléculaire calculée: 448 aa, 50 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC022280
• Symbole du gène: Fibulin 5
• Identification du gène (NCBI): 10516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
mouse	WB,IF	Mater Sci Eng C Mater Biol Appl; Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation.; Authors - Dongdong Yao
human	IHC	PLoS One; Oncogenic Fibulin-5 Promotes Nasopharyngeal Carcinoma Cell Metastasis through the FLJ10540/AKT Pathway and Correlates with Poor Prognosis.; Authors - Chung-Feng Hwang
human	WB	J Biomol Screen; A high-throughput cell-based gaussia luciferase reporter assay for identifying modulators of fibulin-3 secretion.; Authors - Hulleman John D JD
human	WB	Stem Cells Int; Effects of Fibulin-5 Gene Silencing on Proliferation and Apoptosis of IgG4-ROD Lacrimal Gland Fibroblasts; Authors - Huarong Wu
mouse	WB,IP	Free Radic Biol Med; Extracellular SOD modulates canonical TNFα signaling and α5β1 integrin transactivation in vascular smooth muscle cells; Authors - Hyehun Choi ; KD Validated

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