Anticorps Polyclonal de lapin anti-fibrillin 2-Specific

fibrillin 2-Specific Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20252-1-AP

Synonymes

CCA, DA9, FBN2, fibrillin 2, fibrillin 2-Specific

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Galerie de données de validation

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  WB analysis of human brain using 20252-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20252-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20252-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20252-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20252-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20252-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20252-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HepG2, cellules L02, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

20252-1-AP cible fibrillin 2-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: fibrillin 2
• Masse moléculaire calculée: 315 kDa
• Poids moléculaire observé: 290 kDa, 160 kDa
• Numéro d’acquisition GenBank: NM_001999
• Symbole du gène: FBN2
• Identification du gène (NCBI): 2201
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBN2 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils which occur either in association with elastin or in elastin-free bundles. Fibrillin-2-containing microfibrils regulate the early process of elastic fiber assembly. Defects in FBN2 are the cause of congenital contractural arachnodactyly (CCA) which also known as Beals syndrome or distal arthrogryposis type 9 (DA9). This antibody is specific to FBN2. It recognizes both two isoforms ~300 kDa and 160 kDa.

Publications

Species	Application	Title
human	WB	Head Neck; High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas.; Authors - Sreenivasulu Chintala
mouse	IHC	J Ovarian Res; hUMSC transplantation restores follicle development in ovary damaged mice via re-establish extracellular matrix (ECM) components; Authors - Yin Shuyuan