Anticorps Polyclonal de lapin anti-FBX15

• Phare
• Validé par KD/KO

FBX15 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13024-1-AP

Synonymes

FBXO15, F-box only protein 15, F box protein 15, F box only protein 15

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Galerie de données de validation

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  WB analysis of A2780 using 13024-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 13024-1-AP (FBX15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 13024-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13024-1-AP (FBX15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 13024-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13024-1-AP (FBX15 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A2780, tissu ovarien de souris
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

13024-1-AP cible FBX15 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBX15 Protéine recombinante Ag3564
• Nom complet: F-box protein 15
• Masse moléculaire calculée: 434 aa, 49 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC029579
• Symbole du gène: FBX15
• Identification du gène (NCBI): 201456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. The F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. FBOX15 (or FBX15) belongs to the FBXs class, and 2 isoforms produced by alternative splicing has been described. This antibody is expected detect both isoforms.

Publications

Species	Application	Title
mouse	WB	Sci Rep; Oroxylin A alleviates immunoparalysis of CLP mice by degrading CHOP through interacting with FBXO15.; Authors - Zhaoxin Zhang ; KD Validated
human	WB	NPJ Precis Oncol; Targeting USP8 causes synthetic lethality through degradation of FGFR2 in ARID1A-deficient ovarian clear cell carcinoma; Authors - Ryosuke Saito
mouse	WB	Phytomedicine; Gastrodin reduces Aβ brain levels in an Alzheimer's disease mouse model by inhibiting P-glycoprotein ubiquitination; Authors - Chenghao Zhu
human	WB	Clin Transl Med; Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma; Authors - Hao Zhang

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