Anticorps Polyclonal de lapin anti-FBXW2

• Phare
• Validé par KD/KO

FBXW2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 11499-1-AP

Synonymes

F-box and WD-40 domain-containing protein 2, F-box/WD repeat-containing protein 2, FBW2, FBXW 2, FWD2

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Galerie de données de validation

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  WB analysis of HEK-293 using 11499-1-AP

  WB result of FBXW2 antibody (11499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXW2 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 11499-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11499-1-AP (FBXW2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11499-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11499-1-AP (FBXW2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11499-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11499-1-AP (FBXW2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11499-1-AP cible FBXW2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXW2 Protéine recombinante Ag2045
• Nom complet: F-box and WD repeat domain containing 2
• Masse moléculaire calculée: 44 kDa, 52 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC018738
• Symbole du gène: FBXW2
• Identification du gène (NCBI): 26190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human FBW2 polypeptide contains an N-terminal F-box motif and a C-terminal domain of five WD repeats. A WD repeat is a motif typically consisting of 44-60 amino acids with a signature tryptophan and aspartic acid dipeptide at its C-terminus. The WD repeats in FBW2 recognize GCM1 in a way that is facilitated by GSK3b. Two isoforms were produced by alternative splicing with predicted MW of 52 and 43 kDa according to UniProt, and Catalog#11499-1-AP can recognise both.

Publications

Species	Application	Title
human	WB	Nat Commun; The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.; Authors - Jie Xu ; KD Validated
human	WB	Cancer Lett; FBXO43 promotes cell cycle progression in cancer cells through stabilizing SKP2; Authors - Liyun Zheng
human	WB	Cell Mol Gastroenterol Hepatol; TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance.; Authors - Shunjie Xia ; KD Validated
human	WB	Cell Mol Life Sci; FBXW2 inhibits prostate cancer proliferation and metastasis via promoting EGFR ubiquitylation and degradation.; Authors - Tao Zhou ; KD Validated
human	IP	J Biol Chem; USP2 is an SKP2 deubiquitylase that stabilizes both SKP2 and its substrates.; Authors - Fengwu Zhang
human	WB,IHC	Nat Commun; FBXW2 suppresses migration and invasion of lung cancer cells via promoting β-catenin ubiquitylation and degradation.; Authors - Fei Yang ; KD Validated

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