Anticorps Polyclonal de lapin anti-FBXW4

FBXW4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 10657-1-AP

Synonymes

DAC, Dactylin, FBW4, FBWD4, FBXW4, SHFM3, SHSF3

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Galerie de données de validation

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  WB analysis of mouse lung using 10657-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10657-1-AP (FBXW4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10657-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10657-1-AP (FBXW4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 10657-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10657-1-AP (FBXW4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 10657-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10657-1-AP (FBXW4 Antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10657-1-AP cible FBXW4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXW4 Protéine recombinante Ag1047
• Nom complet: F-box and WD repeat domain containing 4
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC007380
• Symbole du gène: FBXW4
• Identification du gène (NCBI): 6468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW4, also known as SHFM3, encodes F-box and WD-40 domain-containing protein 4. Defects in SHFM3 are a cause of split-hand/foot malformation type 3 (SHFM3), an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

wei (Verified Customer) (07-23-2025)

USED WORK WELL

• Applications: Immunohistochemistry, Flow Cytometry

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