Anticorps Polyclonal de lapin anti-FceR1a

FceR1a Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 10980-1-AP

Synonymes

IgE Fc receptor subunit alpha, FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A

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Galerie de données de validation

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  WB analysis of human spleen using 10980-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc epsilon RI alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc Epsilon RI Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 10980-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc epsilon RI alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MC/9, cellules RAW 264.7, tissu splénique de rat, tissu splénique de souris, tissu splénique humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10980-1-AP cible FceR1a dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FceR1a Protéine recombinante Ag1398
• Nom complet: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 55-70 kDa
• Numéro d’acquisition GenBank: BC015195
• Symbole du gène: FceR1a
• Identification du gène (NCBI): 2205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).

Publications

Species	Application	Title
mouse	IF	Science; Response to Comment on "Tumor-initiating cells establish an IL-33-TGF-β niche signaling loop to promote cancer progression".; Authors - Sachiko Taniguchi
human	IHC	J Allergy Clin Immunol; Mast cell-associated alveolar inflammation in patients with atopic uncontrolled asthma.; Authors - Andersson Cecilia K CK
human	WB	J Allergy Clin Immunol; Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2.; Authors - Yunsheng Liang
mouse	WB	Transl Res; Allergic asthma aggravates angiotensin Ⅱ-induced cardiac remodeling in mice.; Authors - Chi Geng ; KO Validated
mouse	WB	Theranostics; Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.; Authors - Hongmei Zhao
human	IHC	Thorax; Novel site-specific mast cell subpopulations in the human lung.; Authors - Andersson C K CK

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