Anticorps Polyclonal de lapin anti-FcRn-Specific

• Phare
• Validé par KD/KO

FcRn-Specific Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16190-1-AP

Synonymes

alpha chain, FCGRT, FCRN, FcRn-Specific, Neonatal Fc receptor

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Galerie de données de validation

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  WB analysis using 16190-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16190-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16190-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules THP-1, tissu hépatique humain, tissu placentaire humain
• Résultats positifs en IHC: tissu splénique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

16190-1-AP cible FcRn-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Fc fragment of IgG, receptor, transporter, alpha
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: NM_004107
• Symbole du gène: FCGRT
• Identification du gène (NCBI): 2217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228).

Publications

Species	Application	Title
human	WB	Cell; Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B.; Authors - Xin Zhao ; KO Validated
pig	WB	Clin Dev Immunol; Human antibodies can cross guinea pig placenta and bind its neonatal Fc Receptor: implications for studying immune prophylaxis and therapy during pregnancy.; Authors - Struble Evi Budo EB
canine	WB,IF	Placenta; Gestation age dependent transfer of human immunoglobulins across placenta in timed-pregnant guinea pigs.; Authors - Yanqun Xu
human	WB	Int J Mol Sci; Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes; Authors - Zhonghao Li

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