Anticorps Polyclonal de lapin anti-FCHSD1

FCHSD1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 23362-1-AP

Synonymes

FCH and double SH3 domains 1, FCHSD1, NWK2

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Galerie de données de validation

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  WB analysis using 23362-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23362-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23362-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23362-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23362-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 23362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 23362-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using FCHSD1 antibody (23362-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

23362-1-AP cible FCHSD1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FCHSD1 Protéine recombinante Ag19963
• Nom complet: FCH and double SH3 domains 1
• Masse moléculaire calculée: 690 aa, 77 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC047016
• Symbole du gène: FCHSD1
• Identification du gène (NCBI): 89848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FCHSD1 (FCH and double SH3 domains 1), also known as NWK2, is a member of the F-BAR family, which is involved in endocytosis and vesicle trafficking. FCHSD1 is expressed in mouse cochlear sensory hair cells and plays an important role in regulating the assembly or maintenance of F-actin in hair cell stereocilia and cuticular plate (PMID: 23437151). FCHSD1 also plays a role in stress response sensing and oxidative stress integration and is involved in the pathogenesis of emphysema (PMID: 34168078). FCHSD1 has three isoforms with molecular weights of 77 kDa, 46 kDa, and 40 kDa.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.; Authors - Takahiro Kawasaki