Anticorps Recombinant de lapin anti-FDX1

• Phare
• Validé par KD/KO

FDX1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230196B12

N° de cat : 82957-1-RR

Synonymes

TMEM79, 230196B12, 230487H5, ADX, FDX

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Galerie de données de validation

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  WB analysis using 82957-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82957-1-RR (FDX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82957-1-RR

  WB result of FDX1 antibody (82957-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HEK-293 cells.

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  WB analysis using 82957-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82957-1-RR (FDX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82957-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82957-1-RR (FDX1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 82957-1-RR

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82957-1-RR (FDX1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82957-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (82957-1-RR, Clone: 230196B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 82957-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human FDX1 (82957-1-RR, Clone:230196B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HepG2 using 82957-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human FDX1 (82957-1-RR, Clone:230196B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82957-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82957-1-RR against Human FDX1 were performed. The affinity constant is 4.02 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HepG2, cellules PC-3
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules A431, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82957-1-RR cible FDX1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FDX1 Protéine recombinante Ag3301
• Nom complet: ferredoxin 1
• Masse moléculaire calculée: 184 aa, 19 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC017063
• Symbole du gène: FDX1
• Identification du gène (NCBI): 2230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

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