Anticorps Polyclonal de lapin anti-FECH

FECH Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(24)

Avis (2)

N° de cat : 14466-1-AP

Synonymes

EC:4.98.1.1, EPP, EPP1, FCE, Protoheme ferro-lyase

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Galerie de données de validation

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  WB analysis using 14466-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14466-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14466-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14466-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14466-1-AP (FECH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 14466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14466-1-AP (FECH Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HepG2, cellules K-562, tissu cardiaque de rat, tissu cardiaque humain, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• IHC: See 2 publications below

Informations sur le produit

14466-1-AP cible FECH dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FECH Protéine recombinante Ag5814
• Nom complet: ferrochelatase (protoporphyria)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 40-48 kDa
• Numéro d’acquisition GenBank: BC039841
• Symbole du gène: FECH
• Identification du gène (NCBI): 2235
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FECH(ferrochelatase, mitochondrial ) is also named as heme synthase, protoheme ferro-lyase and belongs to the ferrochelatase family. It is a homodimeric (86 kDa) mitochondrial membrane-associated enzyme that catalyzes the insertion of ferrous iron into protoporphyrin to form heme(PMID:11175906). It has 2 isoforms produced by alternative splicing and the full length protein has a transit peptide with 54 amino acids.

Publications

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Cancer Discov; NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins; Authors - Naiara Santana-Codina
human	WB	Cell Metab; A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.; Authors - Nunziata Maio
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	J Am Coll Cardiol; Heme levels are increased in human failing hearts.; Authors - Khechaduri Arineh A
mouse	WB	Nat Commun; PMID: 32019928; Authors - Erika M Palmieri

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sofia (Verified Customer) (06-25-2025)

The antibody worked very well in western immunoblotting using 30µg liver lysates of murine origin.

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 800, 5% milk/TBST
• Cell Tissue Type: murine liver whole organ homogenates

FH

Marina (Verified Customer) (07-24-2024)

Incubation with primary antibody ON at 4C. Incubation with secondary antibody (rabbit) for 2h at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human cell lines

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