Anticorps Polyclonal de lapin anti-Kindlin 2

• Phare
• Validé par KD/KO

Kindlin 2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

27

Avis (1)

N° de cat : 11453-1-AP

Synonymes

Fermitin family homolog 2, FERMT2, KIND2, Kindlin 2, mig 2, MIG2, PLEKHC1, UNC112, UNC112B

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Galerie de données de validation

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  WB analysis of A549 using 11453-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11453-1-AP (Kindlin 2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11453-1-AP

  WB result of Kindlin 2 antibody (11453-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Kindlin 2 transfected A549 cells.

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  IP experiment of A549 using 11453-1-AP

  IP Result of anti-FERMT2 (IP:11453-1-AP, 3ug; Detection:11453-1-AP 1:1000) with A549 cells lysate 3300ug.

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  IHC staining of human lung cancer using 11453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11453-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11453-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 22 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• IP: See 4 publications below

Informations sur le produit

11453-1-AP cible Kindlin 2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kindlin 2 Protéine recombinante Ag2013
• Nom complet: fermitin family homolog 2 (Drosophila)
• Masse moléculaire calculée: 680 aa, 78 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC017327
• Symbole du gène: FERMT2
• Identification du gène (NCBI): 10979
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.; Authors - Ling Guo
mouse, zebrafish	WB, IF	Circ Res; Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.; Authors - James J Dowling ; KD Validated
mouse,human	WB,IP	J Am Soc Nephrol; Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.; Authors - Ying Sun ; KD Validated
human	WB, IF	J Cell Sci; Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.; Authors - Clotilde Huet-Calderwood ; KD Validated
human	WB	J Cell Sci; Kindlin-2 promotes rear focal adhesion disassembly and directional persistence in cell migration; Authors - Jie Liu ; KD Validated
human	IF	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yasmin (Verified Customer) (08-01-2019)

Excellent antibody, highly recommended for western blot.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for western blot, 1:100 for IF
• Cell Tissue Type: HeLa, NIH3T3

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