Anticorps Polyclonal de lapin anti-Fibrinogen Beta Chain

• Phare
• Validé par KD/KO

Fibrinogen Beta Chain Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 16747-1-AP

Synonymes

FGB, fibrinogen beta chain

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Galerie de données de validation

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  WB analysis of mouse heart using 16747-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16747-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16747-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16747-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16747-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16747-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cérébral de souris, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

16747-1-AP cible Fibrinogen Beta Chain dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Plasmodium falciparum
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fibrinogen Beta Chain Protéine recombinante Ag10169
• Nom complet: fibrinogen beta chain
• Masse moléculaire calculée: 491 aa, 56 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC107766
• Symbole du gène: FGB
• Identification du gène (NCBI): 2244
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGB is the beta chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide B is cleaved from FGB by thrombin. Mutations in the gene of FGB lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.

Publications

Species	Application	Title
human,rat	IHC,IF	Blood; Fibrinogen β-derived Bβ(15-42) peptide protects against kidney ischemia/reperfusion injury.; Authors - Krishnamoorthy Aparna A
human	WB,IHC,IF	Front Neurosci; Fibrinogen Chains Intrinsic to the Brain.; Authors - Eugene V Golanov ; KD Validated
	WB,IF	Reproduction; Formation of fibrin at sights of conceptus adhesion in the ewe.; Authors - Yuta Matsuno
	WB	Biochem Biophys Res Commun; Characterization of lncRNA functioning in ovine conceptuses and endometria during the peri-implantation period.; Authors - Yuta Matsuno
Plasmodium falciparum	WB	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan
mouse,human	IF	Brain Res; Accelerated accumulation of fibrinogen peptide chains with Aβ deposition in Alzheimer's disease (AD) mice and human AD brains.; Authors - Zhihong Bian

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