Anticorps Polyclonal de lapin anti-FGD2

FGD2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 27068-1-AP

Synonymes

Zinc finger FYVE domain-containing protein 4, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2

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Galerie de données de validation

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  WB analysis of Raji using 27068-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27068-1-AP (FGD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27068-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27068-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27068-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27068-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji,
• Résultats positifs en IHC: tissu d'amygdalite humain, human intrahepatic cholangiocarcinoma tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27068-1-AP cible FGD2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGD2 Protéine recombinante Ag25782
• Nom complet: FYVE, RhoGEF and PH domain containing 2
• Masse moléculaire calculée: 655 aa, 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC023645
• Symbole du gène: FGD2
• Identification du gène (NCBI): 221472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGD2 is a member of the formin family of proteins, which are known for their role in regulating the assembly of actin filaments. Formins are characterized by their ability to nucleate and elongate actin filaments, and they play a crucial role in processes that require dynamic actin structures, such as cell motility and cell division. Mutations or alterations in FGD2 have been associated with certain developmental disorders and diseases. For example, mutations in FGD2 are a cause of faciogenital dysplasia (Aarskog-Scott syndrome), a condition characterized by facial and genital abnormalities and short stature.

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