Anticorps Polyclonal de lapin anti-FGF1

FGF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 17400-1-AP

Synonymes

FGF-1, Heparin-binding growth factor 1, HBGF-1, Fibroblast growth factor 1, Endothelial cell growth factor

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Galerie de données de validation

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  WB analysis using 17400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17400-1-AP (FGF1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17400-1-AP (FGF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spinal cord using 17400-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 17400-1-AP (FGF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens).

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  IHC staining of human breast cancer using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human lung cancer using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 17400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17400-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGF1 antibody (17400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu de moelle épinière de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

17400-1-AP cible FGF1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGF1 Protéine recombinante Ag11366
• Nom complet: fibroblast growth factor 1 (acidic)
• Masse moléculaire calculée: 155 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC032697
• Symbole du gène: FGF1
• Identification du gène (NCBI): 2246
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Neuropharmacology; Non-mitogenic fibroblast growth factor 1 protects against ischemic stroke by regulating microglia/macrophage polarization through Nrf2 and NF-κB pathways.; Authors - Confidence Dordoe
human	WB	Life Sci; A derivant of ginsenoside CK and its inhibitory effect on hepatocellular carcinoma.; Authors - Jingjing Zhang
pig	WB	Physiol Rep; Sitagliptin therapy improves myocardial perfusion and arteriolar collateralization in chronically ischemic myocardium: A pilot study; Authors - Sharif A Sabe
mouse	WB	J Cereb Blood Flow Metab; Saturated fatty acids stimulate cytokine production in tanycytes via the PP2Ac-dependent signaling pathway; Authors - Danyang Liu
mouse	WB,IF	Adv Sci (Weinh); Hepatocyte-Derived FGF1 Alleviates Isoniazid and Rifampicin-Induced Liver Injury by Regulating HNF4α-Mediated Bile Acids Synthesis; Authors - Qian Lin ; KO Validated
mouse	WB	Acta Pharmacol Sin; Madecassoside mitigates acute myocardial infarction injury by activating the PKCB/SPARC signaling pathway; Authors - Peng Wang

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