Anticorps Polyclonal de lapin anti-FGF18

FGF18 Polyclonal Antibody for IF, IHC,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11495-1-AP

Synonymes

FGF 18, FGF18, fibroblast growth factor 18, ZFGF5

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Galerie de données de validation

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  IHC staining of mouse kidney using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11495-1-AP

  Immunofluorescent analysis of HepG2 cells using 11495-1-AP (FGF18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu rénal de souris, tissu cardiaque de souris, tissu embryonnaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11495-1-AP cible FGF18 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGF18 Protéine recombinante Ag2041
• Nom complet: fibroblast growth factor 18
• Masse moléculaire calculée: 207 aa, 24 kDa
• Numéro d’acquisition GenBank: BC006245
• Symbole du gène: FGF18
• Identification du gène (NCBI): 8817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGF18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures.

Publications

Species	Application	Title
mouse	WB, IHC	Front Pharmacol; Suppression of FGF5 and FGF18 Expression by Cholesterol-Modified siRNAs Promotes Hair Growth in Mice.; Authors - Jungang Zhao
human	IHC	J Exp Clin Cancer Res; HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.; Authors - Kai Guo
mouse	WB,IF	Ann Transl Med; Cucurbitacin promotes hair growth in mice by inhibiting the expression of fibroblast growth factor 18; Authors - Keke Guo