Anticorps Polyclonal de lapin anti-Fibrinogen Gamma Chain

• Phare
• Validé par KD/KO

Fibrinogen Gamma Chain Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 15841-1-AP

Synonymes

FGG, fibrinogen gamma chain

---

Galerie de données de validation

• 

  WB analysis of human spleen using 15841-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen Gamma Chain antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15841-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 15841-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 15841-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de souris, tissu hépatique humain, tissu splénique de rat, tissu splénique de souris, tissu splénique humain
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 6 publications below

Informations sur le produit

15841-1-AP cible Fibrinogen Gamma Chain dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fibrinogen Gamma Chain Protéine recombinante Ag8597
• Nom complet: fibrinogen gamma chain
• Masse moléculaire calculée: 453aa,52 kDa; 437aa,50 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007044
• Symbole du gène: FGG
• Identification du gène (NCBI): 2266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

Publications

Species	Application	Title
human,rat	IHC,IF	Blood; Fibrinogen β-derived Bβ(15-42) peptide protects against kidney ischemia/reperfusion injury.; Authors - Krishnamoorthy Aparna A
mouse		J Thromb Haemost; Chronic liver injury drives non-traditional intrahepatic fibrin(ogen) cross-linking via tissue transglutaminase.; Authors - L G Poole
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
rat	IHC	J Neurotrauma; FTY720 Attenuates Neuropathic Pain after Spinal Cord Injury by Decreasing Systemic and Local Inflammation in a Rat Spinal Cord Compression Model.; Authors - Kazuyoshi Yamazaki
human	WB,IHC,IF	Front Neurosci; Fibrinogen Chains Intrinsic to the Brain.; Authors - Eugene V Golanov ; KD Validated
mouse	IHC	Cell Mol Neurobiol; Myelin Debris Impairs Tight Junctions and Promotes the Migration of Microvascular Endothelial Cells in the Injured Spinal Cord.; Authors - Fei Yao

Produits associés

Fibrinogen Gamma Chain Monoclonal antibody

66158-1-Ig

Fibrinogen Beta Chain Polyclonal antibody

16747-1-AP

Fibrinogen Beta Chain Monoclonal antibody

66186-1-Ig

CD41/Integrin Alpha 2B Polyclonal antibody

24552-1-AP

CD61 / Integrin Beta 3 Polyclonal antibody

18309-1-AP

F2 Polyclonal antibody

24295-1-AP