Anticorps Monoclonal anti-Fibrinogen Gamma Chain

Fibrinogen Gamma Chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2B2B8

N° de cat : 66158-1-Ig

Synonymes

FGG, 2B2B8, PRO2061

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Galerie de données de validation

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  WB analysis of L02 using 66158-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood leukocyte using 66158-1-Ig

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 66158-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50.

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  IHC staining of human kidney using 66158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50.

• 

  IF Staining of L02 using 66158-1-Ig

  Immunofluorescent analysis of L02 cells using 66158-1-Ig (Fibrinogen gamma chain antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: leucocyte de sang périphérique humain, cellules L02, plaquettes de sang périphérique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

66158-1-Ig cible Fibrinogen Gamma Chain dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fibrinogen Gamma Chain Protéine recombinante Ag8817
• Nom complet: fibrinogen gamma chain
• Masse moléculaire calculée: 453aa,52 kDa; 437aa,50 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007044
• Symbole du gène: Fibrinogen Gamma Chain
• Identification du gène (NCBI): 2266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

Publications

Species	Application	Title
human	IHC	Front Genet; Elevated Serum FGG Levels Prognosticate and Promote the Disease Progression in Prostate Cancer.; Authors - H H Peng
		Mol Ther Nucleic Acids; Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.; Authors - Fanfan Li

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