Anticorps Polyclonal de lapin anti-FH

FH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10966-1-AP

Synonymes

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 10966-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10966-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10966-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10966-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10966-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10966-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10966-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10966-1-AP

  IP Result of anti-FH (IP:10966-1-AP, 4ug; Detection:10966-1-AP 1:1000) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human prostate cancer using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10966-1-AP (FH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of HepG2 using 10966-1-AP

  Immunofluorescent analysis of HepG2 cells, using FH antibody 10966-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 10966-1-AP

  Immunofluorescent analysis of HepG2 cells using 10966-1-AP (FH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10966-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug FH antibody (10966-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HepG2, cellules L02, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10966-1-AP cible FH dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FH Protéine recombinante Ag1437
• Nom complet: fumarate hydratase
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC017444
• Symbole du gène: FH
• Identification du gène (NCBI): 2271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts a tumor suppressor functions of FH in sporadic kidney cancer(PMID:21695080). This protein has 2 isoforms produced by alternative initiation.

Publications

Species	Application	Title
human	WB	Oncotarget; Identification of SPOP related metabolic pathways in prostate cancer.; Authors - Min Yan
human	IF	Endocr Relat Cancer; Cellular kinetics of MED12-mutant uterine leiomyoma growth and regression in vivo.; Authors - Vanida Ann Serna
human,mouse	WB,IHC	Pharmaceuticals (Basel); Identification of a Difluorinated Alkoxy Sulfonyl Chloride as a Novel Antitumor Agent for Hepatocellular Carcinoma through Activating Fumarate Hydratase Activity; Authors - Jin Jin