Anticorps Polyclonal de lapin anti-FHL3

• Phare
• Validé par KD/KO

FHL3 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 11028-2-AP

Synonymes

FHL 3, FHL3, four and a half LIM domains 3, Skeletal muscle LIM protein 2, SLIM 2, SLIM2

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Galerie de données de validation

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  WB analysis of K-562 using 11028-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11028-2-AP (FHL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 11028-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11028-2-AP (FHL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11028-2-AP

  IP Result of anti-FHL3 (IP:11028-2-AP, 4ug; Detection:11028-2-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 11028-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11028-2-AP (FHL3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11028-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11028-2-AP (FHL3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu ovarien de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

11028-2-AP cible FHL3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FHL3 Protéine recombinante Ag1495
• Nom complet: four and a half LIM domains 3
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC011697
• Symbole du gène: FHL3
• Identification du gène (NCBI): 2275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Four and a half LIM domain (FHL) protein 3 is a member of the FHL protein family that has roles in the regulation of signal transduction, survival, cell adhesion, and mobility. It also involves in the development and progression of liver cancer. The FHL proteins have been shown to regulate a variety of transcription factors, including SMAD proteins, β-catenin, SRF, AP-1, NFAT, FOXO1, MyoD, and the androgen receptor. Beside, the FHL proteins may involve in muscle growth and differentiation. Recent research revealed FHL3 was a PCBP2 target, also involved in the growth and induced apoptosis of glioma cell (23585479). This antibody recognizes specifically the 34kd FHL3 protein.

Publications

Species	Application	Title
human	WB,IHC	J Clin Invest; RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3.; Authors - Han Wei W
human	WB	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu
human	WB,IP	Cell Death Differ; FHL3 links cell growth and self-renewal by modulating SOX4 in glioma.; Authors - Wei Han ; KD Validated
human	WB,IHC,IF	Aging (Albany NY); FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors.; Authors - Pengping Li ; KD Validated
human	WB	J Virol; The four and a half LIM family members are novel interactants of the human T-cell leukemia virus type 1 Tax oncoprotein.; Authors - McCabe áine á
human	IF	Front Oncol; FHL3 Contributes to EMT and Chemotherapy Resistance Through Up-Regulation of Slug and Activation of TGFβ/Smad-Independent Pathways in Gastric Cancer.; Authors - Guodong Cao