Anticorps Recombinant de lapin anti-FITC

FITC Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

FITC conjugated et plus (1)

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1B2

N° de cat : 80003-1-RR

Synonymes

5-Isothiocyanat Fluorescein, Fluorescein

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Galerie de données de validation

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  WB analysis using 80003-1-RR

  FITC-conjugated secondary antibodies were subjected to SDS PAGE followed by western blot with 80003-1-RR (FITC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: FITC conjugated antbody

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

80003-1-RR cible FITC dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons FITC conjugated

• Réactivité: FITC conjugated
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: FITC
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This rabbit recombinant antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Publications

Species	Application	Title
	IF	Drug Dev Res; Carnosol alleviates sevoflurane-induced cognitive dysfunction by mediating NF-κB pathway in aged rats; Authors - Qing Li
human	IP	Biomed Pharmacother; Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma; Authors - Jialiang Luo

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