Anticorps Polyclonal de lapin anti-FKBP10/FKBP65

• Phare
• Validé par KD/KO

FKBP10/FKBP65 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 12172-1-AP

Synonymes

65 kDa FK506 binding protein, 65 kDa FKBP, FK506 binding protein 10, FKBP 10, FKBP 65, FKBP10, FKBP6, FKBP65, hFKBP65, Immunophilin FKBP65, PPIase FKBP10, Rotamase

---

Galerie de données de validation

• 

  WB analysis using 12172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12172-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12172-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12172-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 12172-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12172-1-AP

  IP Result of anti-FKBP65 (IP:12172-1-AP, 4ug; Detection:12172-1-AP 1:600) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human ovary tumor using 12172-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12172-1-AP (FKBP65 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12172-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12172-1-AP (FKBP65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules NIH/3T3, tissu cérébral humain fœtal, tissu placentaire humain, tissu rénal humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Informations sur le produit

12172-1-AP cible FKBP10/FKBP65 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FKBP10/FKBP65 Protéine recombinante Ag2814
• Nom complet: FK506 binding protein 10, 65 kDa
• Masse moléculaire calculée: 582 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC016467
• Symbole du gène: FKBP10
• Identification du gène (NCBI): 60681
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.; Authors - Victoria Hung
human,mouse	WB	Sci Adv; Melanoma-intrinsic NR2F6 activity regulates antitumor immunity; Authors - Hyungsoo Kim ; KD Validated
human	WB,IF	Proc Natl Acad Sci U S A; Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2.; Authors - Rutger A F Gjaltema ; KD Validated
mouse	IHC	Proc Natl Acad Sci U S A; Localized chondro-ossification underlies joint dysfunction and motor deficits in the Fkbp10 mouse model of osteogenesis imperfecta.; Authors - Joohyun Lim
mouse	WB,IF	Hum Mol Genet; Connective Tissue Alterations in Fkbp10-/- mice.; Authors - Caressa D Lietman
human	WB, IF	Hum Mol Genet; HSP47 and FKBP65 cooperate in the synthesis of type I procollagen.; Authors - Ivan Duran

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Doug (Verified Customer) (10-08-2019)

Excellent antibody for western blot.Validated by shFKBP10.

• Applications: Western Blot,
• Primary Antibody Dilution: 1000X
• Cell Tissue Type: Cell line

FKBP10/FKBP65 Antibody Western Blot, validation (1000X dilution) in Cell line (Cat no:12172-1-AP)