Anticorps Polyclonal de lapin anti-FLCN

• Phare
• Validé par KD/KO

FLCN Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11236-2-AP

Synonymes

BHD, FLCL, FLCN, folliculin

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Galerie de données de validation

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  WB analysis using 11236-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11236-2-AP (FLCN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11236-2-AP

  WB result of FLCN antibody (11236-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLCN transfected HeLa cells.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules NCCIT

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11236-2-AP cible FLCN dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FLCN Protéine recombinante Ag1746
• Nom complet: folliculin
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 64-75 kDa
• Numéro d’acquisition GenBank: BC015687
• Symbole du gène: FLCN
• Identification du gène (NCBI): 201163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma.

Publications

Species	Application	Title
mouse	WB,IP	FASEB J; AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR.; Authors - Caterina Collodet
mouse	WB	DNA Repair (Amst); Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA.; Authors - Lim Teik How TH ; KD Validated
human	IHC	Front Mol Biosci; FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma.; Authors - Xuyang Zhao
	WB	J Trace Elem Med Biol; Methionine-chelated Zn promotes anabolism by integrating mTOR signal and autophagy pathway in juvenile yellow catfish.; Authors - Kun Wu
human	WB	Biochem Biophys Res Commun; Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O?-methylguanine-induced apoptosis.; Authors - Sano Shiori S ; KD Validated
rat	WB	Curr Res Neurobiol; Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models; Authors - Mira Seidel

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