Anticorps Polyclonal de lapin anti-FLCN

FLCN Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 11236-2-AP

Synonymes

BHD, Birt-Hogg-Dube syndrome protein, FLCL, Folliculin

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Galerie de données de validation

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  WB analysis using 11236-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11236-2-AP (FLCN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 11236-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11236-2-AP (FLCN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules NCCIT
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11236-2-AP cible FLCN dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FLCN Protéine recombinante Ag1746
• Nom complet: folliculin
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 64-75 kDa
• Numéro d’acquisition GenBank: BC015687
• Symbole du gène: FLCN
• Identification du gène (NCBI): 201163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma.

Publications

Species	Application	Title
mouse	WB,IP	FASEB J; AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR.; Authors - Caterina Collodet
mouse	WB	DNA Repair (Amst); Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA.; Authors - Lim Teik How TH ; KD Validated
human	IHC	Front Mol Biosci; FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma.; Authors - Xuyang Zhao
	WB	J Trace Elem Med Biol; Methionine-chelated Zn promotes anabolism by integrating mTOR signal and autophagy pathway in juvenile yellow catfish.; Authors - Kun Wu
human	WB	Biochem Biophys Res Commun; Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O?-methylguanine-induced apoptosis.; Authors - Sano Shiori S ; KD Validated
rat	WB	Curr Res Neurobiol; Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models; Authors - Mira Seidel

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