Anticorps Polyclonal de lapin anti-FLI1

• Phare
• Validé par KD/KO

FLI1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11347-1-AP

Synonymes

EWSR2, FLI1, Proto oncogene Fli 1, SIC 1, Transcription factor ERGB

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Galerie de données de validation

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  WB analysis using 11347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11347-1-AP (FLI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11347-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11347-1-AP (FLI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11347-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11347-1-AP (FLI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11347-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11347-1-AP (FLI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Jurkat, cellules MOLT-4, cellules Ramos, tissu cérébral humain, tissu de thymus de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

11347-1-AP cible FLI1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FLI1 Protéine recombinante Ag1899
• Nom complet: Friend leukemia virus integration 1
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC010115
• Symbole du gène: FLI1
• Identification du gène (NCBI): 2313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.

Publications

Species	Application	Title
human,mouse	WB	Mol Ther; Suppression of Fli-1 protects against pericyte loss and cognitive deficits in Alzheimer's disease.; Authors - Pengfei Li
human	IHC	Mol Ther Nucleic Acids; circCAMSAP1 promotes osteosarcoma progression and metastasis by sponging miR-145-5p and regulating FLI1 expression.; Authors - Zizheng Chen
human	WB	Front Cell Infect Microbiol; Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.; Authors - Fengxue Geng
human	WB	Cancer; Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene.; Authors - Zhang Jianjun J ; KD Validated
human	WB	Mol Med Rep; Fli‑1 promotes metastasis by regulating MMP2 signaling in hepatocellular carcinoma.; Authors - Huiling Wang ; KD Validated