Anticorps Polyclonal de lapin anti-FLVCR1

• Phare
• Validé par KD/KO

FLVCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 26841-1-AP

Synonymes

Heme transporter FLVCR1, FLVCR, Feline leukemia virus subgroup C receptor-related protein 1

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Galerie de données de validation

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  WB analysis of Jurkat using 26841-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 26841-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26841-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26841-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 26841-1-AP

  IP result of anti-FLVCR1 (IP:26841-1-AP, 4ug; Detection:26841-1-AP 1:500) with K-562 cells lysate 4400 ug.

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  IHC staining of human breast cancer using 26841-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26841-1-AP (FLVCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 26841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26841-1-AP (FLVCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 26841-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using 26841-1-AP (FLVCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, cellules K-562, tissu placentaire humain
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

26841-1-AP cible FLVCR1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FLVCR1 Protéine recombinante Ag25220
• Nom complet: feline leukemia virus subgroup C cellular receptor 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa~70 kDa
• Numéro d’acquisition GenBank: BC048312
• Symbole du gène: FLVCR1
• Identification du gène (NCBI): 28982
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FLVCR1(feline leukemia virus subgroup C receptor 1) has been reported to have a crucial role in a variety of biological processes, including cell proliferation, cell death, apoptosis, oxidative stress response, cellular differentiation, and metabolism (PMID: 29532854). In addition, FLVCR1 can be applied as a clinical prognostic marker for patients with ESCC (PMID: 33842377).

Publications

Species	Application	Title
mouse	WB	J Nanobiotechnology; Apoptotic metabolites ameliorate bone aging phenotypes via TCOF1/FLVCR1-mediated mitochondrial homeostasis; Authors - Yan Qu ; KD Validated
		Cell Rep; Flvcr1a deficiency promotes heme-based energy metabolism dysfunction in skeletal muscle; Authors - Miriam Mistretta
human,mouse	WB,IP,IF	Cell Rep Med; Dysregulation of FLVCR1a-dependent mitochondrial calcium handling in neural progenitors causes congenital hydrocephalus; Authors - Francesca Bertino ; KD Validated
human	WB	Biochim Biophys Acta Mol Basis Dis; The role of FLVCR1 and FLVCR2 in choline transport in the Caco-2 intestinal epithelial cell model and rat small intestine; Authors - Tomoya Yasujima

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