Anticorps Monoclonal anti-FNTB

• Phare
• Validé par KD/KO

FNTB Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1F3B10

N° de cat : 66783-1-Ig

Synonymes

1F3B10, EC:2.5.1.58, FPTB, FTase beta, FTase-beta

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Galerie de données de validation

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  WB analysis using 66783-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66783-1-Ig

  WB result of FNTB antibody (66783-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected HeLa cells.

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  WB analysis of HEK-293 using 66783-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66783-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 66783-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FNTB antibody (66783-1-Ig, Clone: 1F3B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of A431 using 66783-1-Ig

  1x10^6 A431 cells were intracellularly stained with 0.8 ug FNTB Monoclonal antibody (66783-1-Ig, Clone:1F3B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66783-1-Ig cible FNTB dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FNTB Protéine recombinante Ag27796
• Nom complet: farnesyltransferase, CAAX box, beta
• Masse moléculaire calculée: 437 aa, 49 kDa
• Poids moléculaire observé: 44-45 kDa
• Numéro d’acquisition GenBank: BC020232
• Symbole du gène: FNTB
• Identification du gène (NCBI): 2342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

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