Anticorps Monoclonal anti-FOLR1

• Phare
• Validé par KD/KO

FOLR1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2B4B7

N° de cat : 60307-1-Ig

Synonymes

2B4B7, Adult folate binding protein, Adult folate-binding protein, FBP, Folate receptor 1

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Galerie de données de validation

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  WB analysis of HeLa using 60307-1-Ig

  WB result of FOLR1 antibody (60307-1-Ig, 1:4000) with si-Control and si-FOLR1 transfected HeLa cells..

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  WB analysis of human milk using 60307-1-Ig

  human milk were subjected to SDS PAGE followed by western blot with 60307-1-Ig (FOLR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60307-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60307-1-Ig (FOLR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60307-1-Ig (FOLR1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 60307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60307-1-Ig (FOLR1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: lait humain, cellules HeLa
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

60307-1-Ig cible FOLR1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FOLR1 Protéine recombinante Ag19959
• Nom complet: folate receptor 1 (adult)
• Masse moléculaire calculée: 257 aa, 30 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: BC002947
• Symbole du gène: FOLR1
• Identification du gène (NCBI): 2348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).

Publications

Species	Application	Title
human	WB	ACS Nano; High-Performance Self-Cascade Pyrite Nanozymes for Apoptosis-Ferroptosis Synergistic Tumor Therapy.; Authors - Xiangqin Meng
human	WB	Biosensors (Basel); Folic Acid-Modified Fluorescent-Magnetic Nanoparticles for Efficient Isolation and Identification of Circulating Tumor Cells in Ovarian Cancer.; Authors - Yue Pan
human	WB	Antibodies (Basel); Novel Anti-FOLR1 Antibody-Drug Conjugate MORAb-202 in Breast Cancer and Non-Small Cell Lung Cancer Cells.; Authors - Yuki Matsunaga ; KD Validated
		J Mol Diagn; Colocalization of cancer-associated biomarkers on single extracellular vesicles for early detection of cancer; Authors - Daniel P Salem
human	IHC	Arch Pathol Lab Med; Evaluation of Laboratory-Derived Immunohistochemical Assays for Folate Receptor α Expression in Epithelial Ovarian Cancer and Comparison With a Companion Diagnostic; Authors - Emily Deutschman
human	IHC	Cells; Mirvetuximab Soravtansine Induces Potent Cytotoxicity and Bystander Effect in Cisplatin-Resistant Germ Cell Tumor Cells; Authors - Lucia Kucerova

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