Anticorps Polyclonal de lapin anti-FOXC1

• Phare
• Validé par KD/KO

FOXC1 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 55365-1-AP

Synonymes

ARA, FKHL7, forkhead box C1, Forkhead box protein C1, Forkhead related protein FKHL7, FOXC1, FREAC 3, FREAC3, IGDA, IHG1, IRID1, RIEG3

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Galerie de données de validation

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  WB analysis of HEK-293 using 55365-1-AP

  WB result of FOXC1 antibody (55365-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXC1 transfected HEK-293 cells.

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  WB analysis of MCF-7 using 55365-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55365-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55365-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55365-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 55365-1-AP

  IP result of anti-FOXC1 (IP:55365-1-AP, 4ug; Detection:55365-1-AP 1:300) with HepG2 cells lysate 4000 ug.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55365-1-AP cible FOXC1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: forkhead box C1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_001453
• Symbole du gène: FOXC1
• Identification du gène (NCBI): 2296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly. This antibody is specific to FOXC1. Phosphorylation modification of Foxc1 protein may be responsible for the larger molecular weight of detection compared with theoretical molecular weight (PMID:27708239;16403239).

Publications

Species	Application	Title
human	WB	Bone; The osteoporosis risk variant rs9820407 at 3p22.1 acts as an allele-specific enhancer to regulate CTNNB1 expression by long-range chromatin loop formation.; Authors - Ya Wang ; KD Validated
human	WB	Tumour Biol; The long noncoding RNA FOXCUT promotes proliferation and migration by targeting FOXC1 in nasopharyngeal carcinoma.; Authors - Yu-Zhong Xu ; KD Validated
human	WB	Mol Med Rep; FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling.; Authors - Qinchen Cao ; KD Validated
human	WB,IHC,CoIP	Phytomedicine; Icaritin inhibits endometrial carcinoma cells by suppressing O-GlcNAcylation of FOXC1; Authors - Yufei Wang
human	IHC,IF	J Oral Pathol Med; Single-cell RNA sequencing reveals tumor heterogeneity within salivary gland pleomorphic adenoma: A preliminary study; Authors - Xi-Qian Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (08-20-2021)

I was sent 4 antibodies to try. You can see that JUN (1:1000) and NFKB1 (1:1000) worked, but FOXC1 (1:300) and HES1 (1:500) did not. I have shown that the cells express the mRNA for these proteins by qPCR.

• Applications: Western Blot
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Tc28a2 immortalised chondrocytes

FOXC1 Antibody Western Blot validation (1:300 dilution) in Tc28a2 immortalised chondrocytes (Cat no:55365-1-AP)