Anticorps Monoclonal anti-FOXC2

FOXC2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3E3E1

N° de cat : 68384-1-Ig

Synonymes

FKHL14, Forkhead box protein C2, FOXC2, LD, Mesenchyme fork head protein 1, MFH 1, MFH 1 protein, MFH1, Transcription factor FKH 14

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Galerie de données de validation

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  WB analysis of hTERT-RPE1 using 68384-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68384-1-Ig (FOXC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68384-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68384-1-Ig (FOXC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 68384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68384-1-Ig (FOXC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 68384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68384-1-Ig (FOXC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules hTERT-RPE1, cellules MG U-87, tissu adipeux de porc, tissu cardiaque de rat, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

68384-1-Ig cible FOXC2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FOXC2 Protéine recombinante Ag19378
• Nom complet: forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• Masse moléculaire calculée: 501 aa, 54 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC113437
• Symbole du gène: FOXC2
• Identification du gène (NCBI): 2303
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708)

Publications

Species	Application	Title
human	IF	Cell Rep; Stabilizing vimentin phosphorylation inhibits stem-like cell properties and metastasis of hybrid epithelial/mesenchymal carcinomas; Authors - Nick A Kuburich