• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-FOXM1

FOXM1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, CoIP, chIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(98)

N° de cat : 13147-1-AP

Synonymes

FKHL16, Forkhead-related protein FKHL16, HFH11, HFH-11, HNF-3/fork-head homolog 11



Applications testées

Résultats positifs en WBcellules HT-29, cellules A431, cellules HEK-293, cellules HeLa, cellules L02, cellules MCF-7, tissu de côlon de souris, tissu de thymus de souris, tissu splénique de souris, tissu testiculaire de souris, tissu testiculaire humain
Résultats positifs en IPcellules L02,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13147-1-AP cible FOXM1 dans les applications de WB, IP, CoIP, chIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène FOXM1 Protéine recombinante Ag3729
Nom complet forkhead box M 1
Masse moléculaire calculée 83 kDa
Poids moléculaire observé 84-110 kDa
Numéro d’acquisition GenBankBC006192
Symbole du gène FOXM1
Identification du gène (NCBI) 2305
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

Protocole

Product Specific Protocols
WB protocol for FOXM1 antibody 13147-1-APDownload protocol
IP protocol for FOXM1 antibody 13147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cancer Discov

Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment.

Authors - Bo Hu
  • KD Validated
humanWB

Nat Commun

K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis

Authors - Xiao-Lan Zhang
mouseWB

Acta Pharm Sin B

Constitutive androstane receptor induced-hepatomegaly and liver regeneration is partially via yes-associated protein activation.

Authors - Yue Gao
mouseWB

Nat Commun

Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation.

Authors - Tomohito Izumi
humanWB

Nat Commun

FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence.

Authors - Joana Catarina Macedo
humanWB

Nat Commun

circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1.

Authors - Zhuoan Cheng
  • KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jordan (Verified Customer) (11-07-2024)

Works well at 1:1000 overnight at 4c

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
FH

Angie (Verified Customer) (08-27-2024)

A549 lysate was subjected to western blot with FOXM1 antibody used at 1:10000 and incubated at room temperature for 1.5 hours. Secondary antibody: Donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution incubated at room temperature for 1 hour. Image is shown in grayscale. Several bands were observed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10 000
  • Cell Tissue Type: A549
FH

Kausik (Verified Customer) (02-21-2022)

The antibody worked well with immunoprecipitation and ChIP experiments and we had acceptable results with immunoblotting.

  • Applications: Western Blot, Immunoprecipitation, ChIP
  • Cell Tissue Type: human cancer-derived cell lines
FH

Iram (Verified Customer) (09-17-2020)

FOXM1 antibody giving a very clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 for western
  • Cell Tissue Type: Human colon cancer
FH

Terri (Verified Customer) (11-05-2019)

This antibody distinguishes multiple isoforms of FOXM1.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human mesothelioma and ovarian cell lines
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