Anticorps Polyclonal de lapin anti-NCS1

• Phare
• Validé par KD/KO

NCS1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 10506-2-AP

Synonymes

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

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Galerie de données de validation

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  WB analysis of mouse brain using 10506-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10506-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10506-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10506-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10506-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10506-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10506-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 10506-2-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10506-2-AP

  IP result of anti-NCS1 (IP:10506-2-AP, 4ug; Detection:10506-2-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human cerebellum using 10506-2-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 10506-2-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 10506-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 10506-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse cerebellum using 10506-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 10506-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 10506-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 10506-2-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NCS1 (10506-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A431, cellules HepG2, cellules PC-3, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cervelet humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cervelet de souris,
• Résultats positifs en FC (Intra): cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

10506-2-AP cible NCS1 dans les applications de WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCS1 Protéine recombinante Ag0784
• Nom complet: frequenin homolog (Drosophila)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC004856
• Symbole du gène: NCS1
• Identification du gène (NCBI): 23413
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NCS1 is a member of the neuronal calcium sensor gene family. It regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity.

Publications

Species	Application	Title
mouse	WB	Neuron; NCS-1 in the dentate gyrus promotes exploration, synaptic plasticity, and rapid acquisition of spatial memory.; Authors - Bechara J Saab
mouse	WB	Sci Rep; Self-directed exploration provides a Ncs1-dependent learning bonus.; Authors - Ho-Suk Mun ; KD Validated
mouse	WB,CoIP	Sci Transl Med; Activation of the sigma-1 receptor chaperone alleviates symptoms of Wolfram syndrome in preclinical models; Authors - Lucie Crouzier
human	WB	Elife; A hierarchical pathway for assembly of the distal appendages that organize primary cilia; Authors - Tomoharu Kanie
human	WB	Elife; Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages; Authors - Tomoharu Kanie ; KO Validated

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