Anticorps Polyclonal de lapin anti-Fascin

• Phare
• Validé par KD/KO

Fascin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 14384-1-AP

Synonymes

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FSCN1, HSN, p55, Singed like protein, SNL

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Galerie de données de validation

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  WB analysis using 14384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14384-1-AP (Fascin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14384-1-AP

  WB result of Fascin antibody (14384-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fascin transfected HeLa cells.

• 

  WB analysis of mouse thymus using 14384-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14384-1-AP (Fascin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 14384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 14384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14384-1-AP (Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14384-1-AP (Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 14384-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Fascin (14384-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules NIH/3T3, cellules SH-SY5Y, tissu de thymus de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,
• Résultats positifs en cytométrie: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

14384-1-AP cible Fascin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fascin Protéine recombinante Ag5816
• Nom complet: fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC000521
• Symbole du gène: FSCN1
• Identification du gène (NCBI): 6624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis.; Authors - Mei-Ling Xu
human	WB	Int J Biol Macromol; Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro.; Authors - Ajab Khan
human	WB	Exp Cell Res; Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage.; Authors - Jones Lamptey ; KD Validated
human	WB	J Exp Clin Cancer Res; The role of microRNA-133b and its target gene FSCN1 in gastric cancer.; Authors - Lihua Guo
mouse	WB	Immunol Lett; Yes-associated protein upregulates filopodia formation to promote alveolar epithelial-cell phagocytosis.; Authors - Peiyu Gao ; KD Validated
human	IHC	J Anat; Localization of macrophages and dendritic cells in human thoracic lymph nodes: An immunohistochemical study using surgically obtained specimens; Authors - Masaya Aoki

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval NaCit 0.1M ph6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Retina/Brain

Fascin Antibody Immunohistochemistry, validation (1:100 dilution) in Retina/Brain (Cat no:14384-1-AP)