Anticorps Polyclonal de lapin anti-FUCA1

FUCA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 16420-1-AP

Synonymes

Alpha-L-fucosidase 1, Alpha-L-fucosidase I, Alpha-L-fucoside fucohydrolase 1, EC:3.2.1.51, FUCA

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Galerie de données de validation

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  WB analysis of mouse liver using 16420-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16420-1-AP (FUCA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16420-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16420-1-AP (FUCA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16420-1-AP (FUCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16420-1-AP (FUCA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules COLO 320
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 5 publications below

Informations sur le produit

16420-1-AP cible FUCA1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUCA1 Protéine recombinante Ag9612
• Nom complet: fucosidase, alpha-L- 1, tissue
• Masse moléculaire calculée: 461 aa, 53 kDa
• Poids moléculaire observé: 50-56 kDa
• Numéro d’acquisition GenBank: BC017338
• Symbole du gène: FUCA1
• Identification du gène (NCBI): 2517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).

Publications

Species	Application	Title
human	WB,IHC	Oncotarget; Reduced expression of α-L-Fucosidase-1 (FUCA-1) predicts recurrence and shorter cancer specific survival in luminal B LN+ breast cancer patients.; Authors - Serena Bonin
human	WB,IHC	Oncotarget; Human a-L-fucosidase-1 attenuates the invasive properties of thyroid cancer.; Authors - Giancarlo Vecchio
human	WB,IHC	Gene; Potential predictive value of immune-related genes FUCA1 and NCKAP1L for osteosarcoma metastasis; Authors - Xuan Wang
human	WB	Cancer Genomics Proteomics; High Resolution Proteomic Analysis of the Cervical Cancer Cell Lines Secretome Documents Deregulation of Multiple Proteases.; Authors - Kalliopi I Pappa
human	IHC	Pathol Int; Alpha-L-fucosidase-1 is a diagnostic marker that distinguishes mucoepidermoid carcinoma from squamous cell carcinoma.; Authors - Shoko Ishida
human	IHC	Oncotarget; Proteomics analysis of bladder cancer invasion: Targeting EIF3D for therapeutic intervention.; Authors - Agnieszka Latosinska

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