Anticorps Polyclonal de lapin anti-FUCA2

FUCA2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 15157-1-AP

Synonymes

Alpha L fucosidase 2, FUCA2, fucosidase, alpha L 2, plasma, Plasma alpha L fucosidase, UNQ227/PRO260

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Galerie de données de validation

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  WB analysis of human heart using 15157-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15157-1-AP

  IP result of anti-FUCA2 (IP:15157-1-AP, 4ug; Detection:15157-1-AP 1:300) with mouse heart tissue lysate 4000 ug.

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  IHC staining of human liver using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu d'estomac de rat, tissu d'estomac de souris
• Résultats positifs en IP: tissu cardiaque de souris,
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15157-1-AP cible FUCA2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUCA2 Protéine recombinante Ag7250
• Nom complet: fucosidase, alpha-L- 2, plasma
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC003060
• Symbole du gène: FUCA2
• Identification du gène (NCBI): 2519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gretchen (Verified Customer) (02-01-2019)

I did a 1:2000 dilution in 30ug of lysate protein. The bands were sharp and strong and it took 3 seconds to develop. However, I had a lot of unspecific bands and not only at 86, they were some stronger than others.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Melanoma cells