Anticorps Polyclonal de lapin anti-FUNDC1

• Phare
• Validé par KD/KO

FUNDC1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 28519-1-AP

Synonymes

FUN14 domain containing 1, FUN14 domain-containing protein 1, FUNDC 1

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Galerie de données de validation

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  WB analysis using 28519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28519-1-AP (FUNDC1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28519-1-AP (FUNDC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules C6, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below

Informations sur le produit

28519-1-AP cible FUNDC1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUNDC1 Protéine recombinante Ag29358
• Nom complet: FUN14 domain containing 1
• Masse moléculaire calculée: 155 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC042813
• Symbole du gène: FUNDC1
• Identification du gène (NCBI): 139341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUNDC1 is a novel mitochondrial-associated membrane (MAM) protein, enriched at the MAM by interacting with the ER resident protein CANX (calnexin) under hypoxia. As mitophagy proceeds, it dissociates from CANX and preferably recruits DNM1L/DRP1 to drive mitochondrial fission in response to hypoxic stress.

Publications

Species	Application	Title
mouse	WB	Clin Sci (Lond); Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning; Authors - Limin Shi ; KD Validated
goat	WB	FASEB J; Zearalenone modulates the function of goat endometrial cells via the mitochondrial quality control system; Authors - Guomin Zhang
mouse	WB	Mech Ageing Dev; Deletion of Stimulator of Interferons Genes Aggravated Cardiac Dysfunction in Physiological Aged Mice; Authors - Diansa Gao
human	WB	Commun Biol; Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1; Authors - Shuo Wang
human	WB	J Exp Clin Cancer Res; Synthetic lethality of combined ULK1 defection and p53 restoration induce pyroptosis by directly upregulating GSDME transcription and cleavage activation through ROS/NLRP3 signaling; Authors - Wei Chen
monkey	WB	PLoS Pathog; Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation; Authors - Zhe Sun

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