Anticorps Polyclonal de lapin anti-FUT8

FUT8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 12560-1-AP

Synonymes

Alpha-(1,6)-fucosyltransferase, alpha1 6FucT, Alpha1-6FucT, EC:2.4.1.68, FUT 8

---

Galerie de données de validation

• 

  WB analysis using 12560-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12560-1-AP (FUT8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12560-1-AP (FUT8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12560-1-AP (FUT8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12560-1-AP (FUT8 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12560-1-AP (FUT8 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12560-1-AP (FUT8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12560-1-AP (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 12560-1-AP (FUT8 Antibody) at dilution of 1:50.

• 

  IHC staining of human stomach cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 12560-1-AP (FUT8 Antibody) at dilution of 1:50.

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du poumon humain, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Informations sur le produit

12560-1-AP cible FUT8 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUT8 Protéine recombinante Ag3280
• Nom complet: fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC025385
• Symbole du gène: FUT8
• Identification du gène (NCBI): 2530
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).

Publications

Species	Application	Title
human		Am J Cancer Res; α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC).; Authors - Fengzhou Li
human	IHC	Clin Exp Pharmacol Physiol; MicroRNA-198-5p inhibits the migration and invasion of non-small lung cancer cells by targeting fucosyltransferase 8.; Authors - Siyao Wang
human	IHC	Exp Mol Pathol; cDNA microarray analysis and immunohistochemistry reveal a distinct molecular phenotype in serous endometrial cancer compared to endometrioid endometrial cancer.; Authors - Chen Yonghua Y
human	WB,IHC	Biochem Biophys Rep; Partial silencing of fucosyltransferase 8 gene expression inhibits proliferation of Ishikawa cells, a cell line of endometrial cancer.; Authors - Hana Shimoyama ; KD Validated
mouse	WB	Mol Neurobiol; FUT8 Regulates Cerebellar Neurogenesis and Development Through Maintaining the Level of Neural Cell Adhesion Molecule Cntn2; Authors - Kaiyan Wei

{{ptg:RelatedPrimaryAntibodies}}