Anticorps Polyclonal de lapin anti-FXYD6

• Phare
• Validé par KD/KO

FXYD6 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15805-1-AP

Synonymes

FXYD6, Phosphohippolin

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Galerie de données de validation

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  WB analysis using 15805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15805-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 15805-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15805-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15805-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of SH-SY5Y using 15805-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug FXYD6 antibody (15805-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu testiculaire humain
• Résultats positifs en IHC: tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

15805-1-AP cible FXYD6 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FXYD6 Protéine recombinante Ag8538
• Nom complet: FXYD domain containing ion transport regulator 6
• Masse moléculaire calculée: 95 aa, 11 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC018652
• Symbole du gène: FXYD6
• Identification du gène (NCBI): 53826
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The FXYD family is a group of small single-span transmembrane proteins characterized by a signature sequence containing an FXYD motif, two conserved glycines and a serine residue. Members of the FXYD family, including FXYD1 (phospholemman), FXYD2 (gamma subunit of Na,K-ATPase), FXYD3 (Mat8), FXYD4 (CHIF), FXYD5 (RIC), FXYD6 (phosphohippolin) and FXYD7, are tissue specific regulators of the Na,K-ATPase. FXYD6 is primarily expressed in the brain. It modulates the kinetic activity of Na,K-ATPase and has long-term physiological importance in maintaining cation homeostasis. It may play a role in endolymph composition and has a potential important role in neuronal excitability of the CNS during postnatal development and in the adult brain. On the SDS-PAGE FXYD6 migrates with an apparent molecular weight of approximately 20 kDa, which is larger than the calculated molecular weight of 10.5 kDa (PMID: 15193427; 17209044). The gene encodes FXYD6 is located on chromosome 11q23.3, and it might be a susceptibility gene of schizophrenia.

Publications

Species	Application	Title
mouse	IF	J Am Soc Nephrol; Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.; Authors - Sébastien J Dumas
human	WB,IF	Cancer Med; Identification of FXYD6 as the novel biomarker for glioma based on differential expression and DNA methylation; Authors - Weiliang Hou
human	WB	Biomed Res Int; FXYD6 Regulates Chemosensitivity by Mediating the Expression of Na+/K+-ATPase α1 and Affecting Cell Autophagy and Apoptosis in Colorectal Cancer.; Authors - Wen Luo ; KD Validated

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