Anticorps Monoclonal anti-Fascin

• Phare
• Validé par KD/KO

Fascin Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5G3

N° de cat : CL488-66321

Synonymes

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL488-66321

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66321 (Fascin antibody) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL488-66321

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66321 (Fascin antibody) at dilution of 1:100. .

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  FC experiment of SH-SY5Y using CL488-66321

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Fascin (CL488-66321, Clone:1B5G3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using CL488-66321

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Fascin (CL488-66321, Clone:1B5G3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en FC (Intra): cellules HeLa, cellules SH-SY5Y

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66321 cible Fascin dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fascin Protéine recombinante Ag6074
• Nom complet: fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000521
• Symbole du gène: Fascin
• Identification du gène (NCBI): 6624
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

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