Anticorps Monoclonal anti-Fibronectin

• Phare
• Validé par KD/KO

Fibronectin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1G10F9

N° de cat : CL594-66042

Synonymes

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

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Galerie de données de validation

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  IF Staining of human colon cancer using CL594-66042

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Fibronectin antibody (CL594-66042, Clone: 1G10F9 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-66042

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Fibronectin antibody (CL594-66042, Clone: 1G10F9 ) at dilution of 1:200.

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  IF Staining of NIH/3T3 using CL594-66042

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CL594-66042 (Fibronectin antibody) at dilution of 1:100.

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  FC experiment of HEK-293 using CL594-66042

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Fibronectin (CL594-66042, Clone:1G10F9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du côlon humain
• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66042 cible Fibronectin dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fibronectin Protéine recombinante Ag8016
• Nom complet: fibronectin 1
• Masse moléculaire calculée: 2386 aa, 263 kDa
• Poids moléculaire observé: 263 kDa
• Numéro d’acquisition GenBank: BC005858
• Symbole du gène: Fibronectin
• Identification du gène (NCBI): 2335
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

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