Anticorps Polyclonal de lapin anti-G-CSFR

G-CSFR Polyclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, FC,ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 18310-1-AP

Synonymes

CD114, CSF3R, G CSF R, G CSF receptor, GCSFR, G-CSFR, G-CSF-R

---

Galerie de données de validation

• 

  WB analysis of human placenta using 18310-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18310-1-AP (G-CSFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18310-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18310-1-AP (G-CSFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U-937 using 18310-1-AP

  1X10^6 U-937 cells were stained with 0.2ug G-CSFR antibody (18310-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: tissu placentaire humain
• Résultats positifs en cytométrie: cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

18310-1-AP cible G-CSFR dans les applications de WB, FC,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: colony stimulating factor 3 receptor (granulocyte)
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC053585
• Symbole du gène: CSF3R
• Identification du gène (NCBI): 1441
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

G-CSF-R, also named as CSF3R, CD114 and GCSFR is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. It is a 130 kD to 150 kD glycoprotein single chain receptor which binds and activated by GCSF. In addition it may function in some adhesion or recognition events at the cell surface. It has been classified as a member of the hematopoietic (cytokine) receptor family, cytokine receptor class I, or the gp 130 related cytokine receptor family (although it does not apparently bind to gp 130). GCSF receptors can be found on neutrophils, myeloid leukemia cells that respond to GCSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts. In addition, a soluble form is also expressed. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.

Publications

Species	Application	Title
mouse	WB	Mol Metab; Ovarian cancer modulates the immunosuppressive function of CD11b+Gr1+ myeloid cells via glutamine metabolism.; Authors - Mary P Udumula
human	WB	J Cell Physiol; The overexpression of GRASP might inhibit cell proliferation and invasion in hepatocellular carcinoma.; Authors - Yang Cheng