Anticorps Polyclonal de lapin anti-G6PD

• Phare
• Validé par KD/KO

G6PD Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

36

N° de cat : 25413-1-AP

Synonymes

G6PD, G6PD1

---

Galerie de données de validation

• 

  WB analysis using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25413-1-AP

  WB result of G6PD antibody (25413-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells.

• 

  WB analysis of HeLa using 25413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25413-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25413-1-AP (G6PD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 29 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

25413-1-AP cible G6PD dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: G6PD Protéine recombinante Ag21862
• Nom complet: glucose-6-phosphate dehydrogenase
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC000337
• Symbole du gène: G6PD
• Identification du gène (NCBI): 2539
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
human	WB, IP	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
human	WB	Acta Pharmacol Sin; Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells; Authors - Wa Ding
human	WB	Phytomedicine; Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib; Authors - Chen Yang
human,mouse	WB,IHC	Oxid Med Cell Longev; Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.; Authors - Wen-Chih Liu
human,mouse	WB,IHC,IF	Apoptosis; Role of Escin in breast cancer therapy: potential mechanism for inducing ferroptosis and synergistic antitumor activity with cisplatin; Authors - Chenyuan Li ; KD Validated