Anticorps Monoclonal anti-G6PD

• Phare
• Validé par KD/KO

G6PD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

CloneNo.

2A7B12

N° de cat : 66373-1-Ig

Synonymes

2A7B12, EC:1.1.1.49, G6P, G6PD1, Glucose-6-phosphate 1-dehydrogenase

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Galerie de données de validation

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  WB analysis of HeLa using 66373-1-Ig

  WB result of G6PD antibody (66373-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells.

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  WB analysis of HepG2 using 66373-1-Ig

  Several cell lines were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 66373-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66373-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66373-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66373-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using G6PD antibody (66373-1-Ig, Clone: 2A7B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HeLa using 66373-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66373-1-Ig (G6PD antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules L02, cellules NIH/3T3, tissu splénique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

66373-1-Ig cible G6PD dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: G6PD Protéine recombinante Ag21862
• Nom complet: glucose-6-phosphate dehydrogenase
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC000337
• Symbole du gène: G6PD
• Identification du gène (NCBI): 2539
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse,human	IP,IHC,IF	Cell Metab; Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.; Authors - Yang Zhang
rat,mouse	WB	Redox Biol; The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR.; Authors - Shenglan Zeng
mouse,human	WB,IHC	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge
mouse	WB	Clin Transl Med; SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.; Authors - Tiantong Ou
mouse	WB	Int J Mol Sci; G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle.; Authors - Aiwen Jiang ; KD Validated
human	WB	J Pharmacol Exp Ther; Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.; Authors - Ramon Martinez

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