Anticorps Polyclonal de lapin anti-GABARAPL2-Specific

• Phare
• Validé par KD/KO

GABARAPL2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 18724-1-AP

Synonymes

GABARAPL2, ATG8, FLC3A, GABA(A) receptor-associated protein-like 2, Gamma-aminobutyric acid receptor-associated protein-like 2

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Galerie de données de validation

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  WB analysis of COLO 320 using 18724-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18724-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 18724-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18724-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18724-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 18724-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAPL2-Specific antibody (18724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 18724-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GABARAPL2-Specific Polyclonal antibody (18724-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules PC-3, tissu cérébral de souris, tissu cérébral humain, tissu splénique de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2 traitées à la chloroquine,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18724-1-AP cible GABARAPL2-Specific dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GABA(A) receptor-associated protein-like 2
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC005985
• Symbole du gène: GABARAPL2
• Identification du gène (NCBI): 11345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

Publications

Species	Application	Title
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai ; KO Validated
rat	WB	Autophagy; CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.; Authors - Hyun-Kyu An
human	WB	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely
human	IHC	Comput Biol Med; Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data; Authors - Zehua Wang
human	WB	Cell Death Dis; Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.; Authors - Christos E Zois
human	WB,IHC	Neurobiol Dis; Alteration of autophagosomal proteins in the brain of multiple system atrophy.; Authors - Tanji Kunikazu K

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